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TNF-related weak inducer of apoptosis (TWEAK) is a type II membrane protein of the tumor necrosis factor (TNF) family members, high levels expression in human brain, muscle, heart, spleen, and thymus. TWEAK is a 17.3
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Image Search Results
Journal: Stem Cells Translational Medicine
Article Title: Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell‐Derived Choroidal Endothelium
doi: 10.1002/sctm.16-0399
Figure Lengend Snippet: Identification of CEC differentiation candidates using the Taguchi L12 and factorial elimination screens. (A) : Representative image used to determine the percentage of CA4 + iPSC‐derived CECs. (B) : Taguchi L12 half effect of each factor on the mean of percentage of CA4 + cells. (C) : Taguchi L12 half effect of each factor on the standard deviation of percentage of CA4 + cells. (D) : Mean (+SD) percentage of CA4 + cells elicited by each medium in the factorial elimination screen ( n ≥ 8, ****, p < .0001). (E–J) : Immunohistochemistry showing CTGF (E, G, green) and TWEAKR (H, J, green) expression in adult choroids. Sections were dual labeled with the endothelial cell binding lectin UEA‐I (F, G, I, J, red). All scale bars represent 100 μm. Abbreviations: CC, choriocapillaris; CEC, choroidal endothelial cell; CH, choroidal vasculature; CTGF, connective tissue growth factor; DAPI, 4′,6‐diamidino‐2‐phenylindole; RPE, retinal pigment epithelium; TWEAKR, TNF‐related weak inducer of apoptosis receptor; VEGFB, Vascular endothelial growth factor B.
Article Snippet: In order to perform the Taguchi L12 screen, we obtained recombinant human connective tissue growth factor (CTGF; Peprotech; Cat. No. 120‐19, https://www.peprotech.com/en-US ), human β‐catenin (CTNNB1; Sigma; Cat. No. SRP5172‐50UG), human Src Homology 2 Domain‐Containing Transforming Protein 1 (SHC1; Fitzgerald Industries; Cat. No. H00007453‐P01, https://www.fitzgerald-fii.com/ ), human TNF‐related weak inducer of
Techniques: Derivative Assay, Standard Deviation, Immunohistochemistry, Expressing, Labeling, Binding Assay
Journal: Stem Cells Translational Medicine
Article Title: Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell‐Derived Choroidal Endothelium
doi: 10.1002/sctm.16-0399
Figure Lengend Snippet: CTGF drives iPSC differentiation into CECs. (A) : Mean (±SD; n = 9) percentage of CA4 + cells at varying concentrations of CTGF and TWEAKR. (B–E) : Representative images are provided showing cell morphology and CA4 expression at 0 ng/ml TWEAKR and 0 ng/ml CTGF (B), 0 ng/ml TWEAKR and 50 ng/ml CTGF (C), 10 ng/ml TWEAKR and 0 ng/ml CTGF (D), and 10 ng/ml TWEAKR and 50 ng/ml CTGF (E). (F) : Concentration of endogenously secreted CTGF in culture medium from human iPSCs differentiated into CEC in medium containing 10 ng/ml of TWEAKR alone or with varying concentrations of the inhibitor LY2109761, compared to spontaneously differentiated iPSCs grown in basal medium (control), as detected using ELISA. Shown are the means at each condition, while error bars represent the standard deviation ( n = 2). (G) : Percentage of CA4 + cells in cultures of human iPSCs differentiated using 10 ng/ml of TWEAKR alone or with 5 µM inhibitor (LY2109761), compared to spontaneously differentiated iPSCs grown in basal medium (control). Shown are the means at each condition, while error bars represent the standard error of the mean ( n = 9, **, p < .01, ***, p < .001). All scale bars represent 100 μm. Abbreviations: CEC, choroidal endothelial cell; CTGF, Connective tissue growth factor; DAPI, 4′,6‐diamidino‐2‐phenylindole; TWEAKR, TNF‐related weak inducer of apoptosis receptor.
Article Snippet: In order to perform the Taguchi L12 screen, we obtained recombinant human connective tissue growth factor (CTGF; Peprotech; Cat. No. 120‐19, https://www.peprotech.com/en-US ), human β‐catenin (CTNNB1; Sigma; Cat. No. SRP5172‐50UG), human Src Homology 2 Domain‐Containing Transforming Protein 1 (SHC1; Fitzgerald Industries; Cat. No. H00007453‐P01, https://www.fitzgerald-fii.com/ ), human TNF‐related weak inducer of
Techniques: Expressing, Concentration Assay, Control, Enzyme-linked Immunosorbent Assay, Standard Deviation
Journal: Stem Cells Translational Medicine
Article Title: Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell‐Derived Choroidal Endothelium
doi: 10.1002/sctm.16-0399
Figure Lengend Snippet: RF/6A‐secreted factors of interest
Article Snippet: In order to perform the Taguchi L12 screen, we obtained recombinant human connective tissue growth factor (CTGF; Peprotech; Cat. No. 120‐19, https://www.peprotech.com/en-US ), human β‐catenin (CTNNB1; Sigma; Cat. No. SRP5172‐50UG), human Src Homology 2 Domain‐Containing Transforming Protein 1 (SHC1; Fitzgerald Industries; Cat. No. H00007453‐P01, https://www.fitzgerald-fii.com/ ), human TNF‐related weak inducer of
Techniques: